Chapter 7 Questions
Define V0
What is meant by,i) initial rate
ii) average rate
iii) instantaneous rate
Which rate is the fastest rate?
A graph of volume of hydrogen ( [P] ) Vs time given below for the indicated reaction (graph attached)
Calculate,
i) initial rate
ii) average rate
iii) instantaneous rate at 60sec
Define Vmax
Define KM
Why Michalis plot is not used, instead double reciprocal plot is used to determine KM and Vmax?
Do KM and Vmax get affected by available substrate concentration? Explain.
Do KM and Vmax get affected by available enzyme concentration? Explain.
Why p-nitrophenolphosphate → p-nitrophenol is an ideal reaction for this laboratory assay?
Chapter 8: Product Inhibition of Alkaline Phosphatase
List 4 major types of inhibition modes and clearly indicate the effect on Vmax and KM for each mode?
What is the effect of each of the 4 types of inhibitors on the initial rate of an enzyme catalyzed reaction?
A potent inhibitor effectively inhibits an enzyme catalyzed reaction. What kind of a Ki value you would expect for a potent inhibitor?
Considering PNPP → PNP reaction, would you expect to see more intense or pale color for the reaction that contain the inhibitor? Explain.