Lab record: Unknown identification (Part 1)
Mini-report of Unknown Organism (Part 2) (See page 7)
Name_____________________________
Lab record: Unknown identification
Mini-report of Unknown Organism
PART 1: Unknown identification- Test Records (50 points)
Unknown Number #________________________
Cell morphology and Gram Stain
Gram Stain
Cell Morphology
Arrangement
Color
Interpretation (describe cell wall composistion)
Ex 2-2: Colony Morphology
Colony Description (Record the observations for the basic categories terms of colony morphology)
Category
Description term
Colony Shape
Margin
Elevation
Pigment production(color)
Unknown Cultural Characteristics
Ex 2-4: Growth Pattern in Broth (Record the pattern for your organism and its description)
Ex 2-3: Growth Pattern on Slant (Record the pattern for your organism and its description)
Optimum Growth temperature for Culture # ___________
Presumed Optimum temperature for your bacteria is ———————————–.
Why did you assign this temperature as the optimum?__________________________.
EX: 2-7: Oxygen Requirement (Growth in Fluid Thioglycollate Medium (FTM)
Location of growth in FTM
Category based on growth
Respiration Tests
Ex 5-5: Catalase Test
Result (observation)
Interpretation (meaning of result)
List substrate and products of reaction
Symbol (+/-)
.
EX 5-6: Oxidase Test
Result
Interpretation
Symbol
(+/-)
Ex 5-7: Nitrate Reduction Test
Result
Interpretation
Gas (Y/N)
Reagents added (chemical names)
Color after reagents
Zinc
Added
(Yes/NO)
Color after zinc
Fermentation Tests
Ex 5-3 Phenol Red Sugars
PR Sugar
Results
Interpretation
Symbol
(+/-)
EX 5-4: Methyl Red (MR) and Voges-Proskauer (VP) Tests
Test
Reagents added
(use chemical names)
Result
Interpretation
Symbol (+/-)
MR-Test
VP Test
Hydrolysis Tests
Ex 5-12: Starch Hydrolysis
Result (observation)
Interpretation (explain reaction)
Symbol ( +/-)
Ex: 5-13: Urea Hydrolysis
Result (observation)
Interpretation (explain reaction)
symbol (+/_)
Ex 5-14: Casein Hydrolysis
Result (observation)
Interpretation (explain reaction)
Symbol (+/-)
Ex 5-15: Gelatin Hydrolysis
Result (observation)
Interpretation (explain reaction)
Symbol (+/-)
Ex 5-17 Lipid Hydrolysis
Result (observation)
Interpretation (explain reaction)
Symbol
Other Biochemical Tests
Ex 5-8: Citrate Utilization Test
Result
Interpretation
Symbol (+/-)
Ex 5-11: Phenylalanine Deaminase Test
Reagent added
Result
Interpretation (explain reaction)
symbol (+/-)
EX 5-20: SIM Medium
Test
Result
Interpretation (explain reaction)
Symbol (+/-)
Motility
Sulfur Reduction
Indole Production (reagent added)
Fill out this comparison table for your bacterium. Highlight the test that differ between your results and the standard results from Bergey’s Manual. Include this paper with your final report.
Characteristic
Test results from unknown Bacteria #_____________
Name____________
Characteristics reported in Bergey’s Manual
Name __________
Characteristics reported in Bergey’s Manual
Name ___________
Shape
Gram stain
Optimum growth temperature
Motility
Oxygen Requirement
Glucose fermentation
Lactose fermentation
Mannitol fermentation
Arabinose fermentation
Gelatin hydrolysis
Starch hydrolysis
Casein hydrolysis
Fat hydrolysis
Indole test
MR test
VP test
Citrate utilization
Nitrate reduction
H2S production
Urease production
Catalase production
Oxidase production
Phenylalanine deamination
Lysine decarboxylation (Enterotube)
Ornithine decarboxylation (Enterotube)
Adonitol fermentation (Enterotube)
Arabinose fermentation (Enterotube)
Sorbitol fermentation (Enterotube)
Dulcitol fermentation (Enterotube)
Unknown bacterium #________________ is identified as ________________ ___________________
PART 2: Mini-report of Unknown Organism (50 points)
Please include the following sections. Double-spaced, 12 point font.
Introduction and relevant information about the organism you uncovered (20 points)
2-3 paragraphs long. The section should include several paragraphs of background information (i.e. give some general information about the topic/organism you are investigating). Whole report be written in proper English, correct grammar, and spelling and scientifically accurate. This must be your own work reporting your journey in uncovering the Genus species of your unknown bacterium. Make sure to properly format scientific names.
Results / Conclusion (20 points)
The results section also needs to be written in paragraph form but try not to only have one paragraph, it makes for very difficult reading. In the results section you will summarize the results you obtained based upon the all the tests you performed on your organism. Make sure to include Enterotube II results in this area.
For example: The phenol red carbohydrate tests for fermentation from glucose, lactose, mannitol and arabinose were performed. The lactose and mannitol test was performed on unknown #__ and it showed negative after 48 hours of incubation. Glucose and arabinose were yellow after incubation representing acid was produced lowering the pH of phenol red.
References (10 points)
I also require at least five citations. References should be written following the following format:
Ehrlich, P.R., Raven, P.H. (1969) Differentiation of populations. Science 165: 1228-1232.
References should include lab manual, Bergey’s manual and Enterotube book.
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