{"id":68095,"date":"2021-10-30T17:46:35","date_gmt":"2021-10-30T17:46:35","guid":{"rendered":"https:\/\/papersspot.com\/blog\/2021\/10\/30\/pre-lab-qs\/"},"modified":"2021-10-30T17:46:35","modified_gmt":"2021-10-30T17:46:35","slug":"pre-lab-qs","status":"publish","type":"post","link":"https:\/\/papersspot.com\/blog\/2021\/10\/30\/pre-lab-qs\/","title":{"rendered":"Pre Lab Q&#8217;s &#8211;"},"content":{"rendered":"<p>Chapter 7 Questions <br \/>Define V0 <br \/>What is meant by,i) initial rate <br \/>ii) average rate <br \/>iii) instantaneous rate <br \/>Which rate is the fastest rate? <br \/>A graph of volume of hydrogen ( [P] ) Vs time given below for the indicated reaction (graph attached) <br \/>Calculate, <br \/>i) initial rate <br \/>ii) average rate <br \/>iii) instantaneous rate at 60sec <br \/>Define Vmax <br \/>Define KM <br \/>Why Michalis plot is not used, instead double reciprocal plot is used to determine KM and Vmax? <br \/>Do KM and Vmax get affected by available substrate concentration? Explain. <br \/>Do KM and Vmax get affected by available enzyme concentration? Explain. <br \/>Why p-nitrophenolphosphate \u2192 p-nitrophenol is an ideal reaction for this laboratory assay? <br \/>Chapter 8: Product Inhibition of Alkaline Phosphatase <br \/>List 4 major types of inhibition modes and clearly indicate the effect on Vmax and KM for each mode? <br \/>What is the effect of each of the 4 types of inhibitors on the initial rate of an enzyme catalyzed reaction? <br \/>A potent inhibitor effectively inhibits an enzyme catalyzed reaction. What kind of a Ki value you would expect for a potent inhibitor? <br \/>Considering PNPP \u2192 PNP reaction, would you expect to see more intense or pale color for the reaction that contain the inhibitor? Explain. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chapter 7 Questions Define V0 What is meant by,i) initial rate ii) average rate iii) instantaneous rate Which rate is the fastest rate? A graph of volume of hydrogen ( [P] ) Vs time given below for the indicated reaction (graph attached) Calculate, i) initial rate ii) average rate iii) instantaneous rate at 60sec Define [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[10],"class_list":["post-68095","post","type-post","status-publish","format-standard","hentry","category-research-paper-writing","tag-writing"],"_links":{"self":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/posts\/68095","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/comments?post=68095"}],"version-history":[{"count":0,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/posts\/68095\/revisions"}],"wp:attachment":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/media?parent=68095"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/categories?post=68095"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/tags?post=68095"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}