Objective: To order the components in a cell signaling pathway based on protein mutation data.<\/p>\n
In a hypothetical pathway, Ras, protein X, and protein Y are all required for proper signaling. <\/p>\n
Cells in situation (A) were mutated so they do not express protein X. They still express protein Y.<\/p>\n
Cells in situation (B) were mutated so they do not express protein Y. They still express protein X.<\/p>\n
\u201cOveractive Ras\u201d means that Ras was transfected into the cells and is overexpressed. <\/p>\n
Make an observation and corresponding conclusion for situation (A) and situation (B). Alternatively, state the conclusion based on the evidence.<\/p>\n
Use your conclusions (above) to order the pathway starting with the receptor (RTK). Your pathway must include Ras, protein X and protein Y. <\/p>\n
Why was a signal molecule not necessary to cause a response (\u201csignaling restored\u201d) when Ras was transfected into the cells in situation (A)? <\/p>\n
In the cAMP\/ p-CREB dataset presented in class, what was the condition that did not require a signal molecule to cause a response? (see backside) Explain how the treatment worked without a signal.<\/p>\n","protected":false},"excerpt":{"rendered":"
Objective: To order the components in a cell signaling pathway based on protein mutation data. In a hypothetical pathway, Ras, protein X, and protein Y are all required for proper signaling. Cells in situation (A) were mutated so they do not express protein X. They still express protein Y. Cells in situation (B) were mutated […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[10],"class_list":["post-79107","post","type-post","status-publish","format-standard","hentry","category-research-paper-writing","tag-writing"],"_links":{"self":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/posts\/79107","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/comments?post=79107"}],"version-history":[{"count":0,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/posts\/79107\/revisions"}],"wp:attachment":[{"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/media?parent=79107"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/categories?post=79107"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/papersspot.com\/blog\/wp-json\/wp\/v2\/tags?post=79107"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}