Lab record: Unknown identification (Part 1)<\/p>\n
Mini-report of Unknown Organism (Part 2) (See page 7)<\/p>\n
Name_____________________________<\/p>\n
Lab record: Unknown identification<\/p>\n
Mini-report of Unknown Organism <\/p>\n
PART 1: Unknown identification- Test Records (50 points)<\/p>\n
Unknown Number #________________________<\/p>\n
Cell morphology and Gram Stain<\/p>\n
Gram Stain <\/p>\n
Cell Morphology<\/p>\n
Arrangement<\/p>\n
Color<\/p>\n
Interpretation (describe cell wall composistion)<\/p>\n
Ex 2-2: Colony Morphology<\/p>\n
Colony Description (Record the observations for the basic categories terms of colony morphology)<\/p>\n
Category <\/p>\n
Description term <\/p>\n
Colony Shape<\/p>\n
Margin<\/p>\n
Elevation<\/p>\n
Pigment production(color)<\/p>\n
Unknown Cultural Characteristics<\/p>\n
Ex 2-4: Growth Pattern in Broth (Record the pattern for your organism and its description)<\/p>\n
Ex 2-3: Growth Pattern on Slant (Record the pattern for your organism and its description)<\/p>\n
Optimum Growth temperature for Culture # ___________<\/p>\n
Presumed Optimum temperature for your bacteria is ———————————–.<\/p>\n
Why did you assign this temperature as the optimum?__________________________. <\/p>\n
EX: 2-7: Oxygen Requirement (Growth in Fluid Thioglycollate Medium (FTM)<\/p>\n
Location of growth in FTM<\/p>\n
Category based on growth<\/p>\n
Respiration Tests<\/p>\n
Ex 5-5: Catalase Test<\/p>\n
Result (observation)<\/p>\n
Interpretation (meaning of result)<\/p>\n
List substrate and products of reaction<\/p>\n
Symbol (+\/-)<\/p>\n
.<\/p>\n
EX 5-6: Oxidase Test<\/p>\n
Result <\/p>\n
Interpretation<\/p>\n
Symbol<\/p>\n
(+\/-)<\/p>\n
Ex 5-7: Nitrate Reduction Test<\/p>\n
Result<\/p>\n
Interpretation<\/p>\n
Gas (Y\/N)<\/p>\n
Reagents added (chemical names)<\/p>\n
Color after reagents<\/p>\n
Zinc<\/p>\n
Added<\/p>\n
(Yes\/NO)<\/p>\n
Color after zinc<\/p>\n
Fermentation Tests<\/p>\n
Ex 5-3 Phenol Red Sugars<\/p>\n
PR Sugar<\/p>\n
Results<\/p>\n
Interpretation<\/p>\n
Symbol <\/p>\n
(+\/-)<\/p>\n
EX 5-4: Methyl Red (MR) and Voges-Proskauer (VP) Tests<\/p>\n
Test<\/p>\n
Reagents added <\/p>\n
(use chemical names)<\/p>\n
Result<\/p>\n
Interpretation<\/p>\n
Symbol (+\/-)<\/p>\n
MR-Test<\/p>\n
VP Test<\/p>\n
Hydrolysis Tests<\/p>\n
Ex 5-12: Starch Hydrolysis<\/p>\n
Result (observation)<\/p>\n
Interpretation (explain reaction)<\/p>\n
Symbol ( +\/-)<\/p>\n
Ex: 5-13: Urea Hydrolysis<\/p>\n
Result (observation)<\/p>\n
Interpretation (explain reaction)<\/p>\n
symbol (+\/_)<\/p>\n
Ex 5-14: Casein Hydrolysis<\/p>\n
Result (observation)<\/p>\n
Interpretation (explain reaction)<\/p>\n
Symbol (+\/-)<\/p>\n
Ex 5-15: Gelatin Hydrolysis<\/p>\n
Result (observation)<\/p>\n
Interpretation (explain reaction)<\/p>\n
Symbol (+\/-)<\/p>\n
Ex 5-17 Lipid Hydrolysis <\/p>\n
Result (observation)<\/p>\n
Interpretation (explain reaction)<\/p>\n
Symbol<\/p>\n
Other Biochemical Tests<\/p>\n
Ex 5-8: Citrate Utilization Test<\/p>\n
Result<\/p>\n
Interpretation <\/p>\n
Symbol (+\/-)<\/p>\n
Ex 5-11: Phenylalanine Deaminase Test<\/p>\n
Reagent added <\/p>\n
Result<\/p>\n
Interpretation (explain reaction)<\/p>\n
symbol (+\/-)<\/p>\n
EX 5-20: SIM Medium<\/p>\n
Test<\/p>\n
Result<\/p>\n
Interpretation (explain reaction)<\/p>\n
Symbol (+\/-)<\/p>\n
Motility<\/p>\n
Sulfur Reduction<\/p>\n
Indole Production (reagent added)<\/p>\n
Fill out this comparison table for your bacterium. Highlight the test that differ between your results and the standard results from Bergey’s Manual. Include this paper with your final report. <\/p>\n
Characteristic<\/p>\n
Test results from unknown Bacteria #_____________<\/p>\n
Name____________<\/p>\n
Characteristics reported in Bergey\u2019s Manual<\/p>\n
Name __________<\/p>\n
Characteristics reported in Bergey\u2019s Manual <\/p>\n
Name ___________<\/p>\n
Shape<\/p>\n
Gram stain<\/p>\n
Optimum growth temperature<\/p>\n
Motility<\/p>\n
Oxygen Requirement<\/p>\n
Glucose fermentation<\/p>\n
Lactose fermentation<\/p>\n
Mannitol fermentation<\/p>\n
Arabinose fermentation<\/p>\n
Gelatin hydrolysis<\/p>\n
Starch hydrolysis<\/p>\n
Casein hydrolysis<\/p>\n
Fat hydrolysis<\/p>\n
Indole test<\/p>\n
MR test<\/p>\n
VP test<\/p>\n
Citrate utilization<\/p>\n
Nitrate reduction<\/p>\n
H2S production<\/p>\n
Urease production<\/p>\n
Catalase production<\/p>\n
Oxidase production<\/p>\n
Phenylalanine deamination<\/p>\n
Lysine decarboxylation (Enterotube)<\/p>\n
Ornithine decarboxylation (Enterotube)<\/p>\n
Adonitol fermentation (Enterotube)<\/p>\n
Arabinose fermentation (Enterotube)<\/p>\n
Sorbitol fermentation (Enterotube)<\/p>\n
Dulcitol fermentation (Enterotube)<\/p>\n
Unknown bacterium #________________ is identified as ________________ ___________________<\/p>\n
PART 2: Mini-report of Unknown Organism (50 points)<\/p>\n
Please include the following sections. Double-spaced, 12 point font.<\/p>\n
Introduction and relevant information about the organism you uncovered (20 points)<\/p>\n
2-3 paragraphs long. The section should include several paragraphs of background information (i.e. give some general information about the topic\/organism you are investigating). Whole report be written in proper English, correct grammar, and spelling and scientifically accurate. This must be your own work reporting your journey in uncovering the Genus species of your unknown bacterium. Make sure to properly format scientific names.<\/p>\n
Results \/ Conclusion (20 points)<\/p>\n
The results section also needs to be written in paragraph form but try not to only have one paragraph, it makes for very difficult reading. In the results section you will summarize the results you obtained based upon the all the tests you performed on your organism. Make sure to include Enterotube II results in this area. <\/p>\n
For example: The phenol red carbohydrate tests for fermentation from glucose, lactose, mannitol and arabinose were performed. The lactose and mannitol test was performed on unknown #__ and it showed negative after 48 hours of incubation. Glucose and arabinose were yellow after incubation representing acid was produced lowering the pH of phenol red.<\/p>\n
References (10 points)<\/p>\n
I also require at least five citations. References should be written following the following format:<\/p>\n
Ehrlich, P.R., Raven, P.H. (1969) Differentiation of populations. Science 165: 1228-1232.<\/p>\n
References should include lab manual, Bergey\u2019s manual and Enterotube book.<\/p>\n
1<\/p>\n
6<\/p>\n","protected":false},"excerpt":{"rendered":"
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