We changed the heat shock to 30 seconds instead of 90 seconds.
It didn’t work because we didn’t see any colonies on the plates.
LB- one lawn plus 17 colonies
LB 8 colonies
LB/AMP no colonies
LB/AMP- no colonies
Hypothesis: reducing the heat shock time from 90 seconds to 30 seconds will produce more ecoli and all plates will have more growth.
Null hypothesis: reducing the heat shock time from 90 seconds to 30 seconds will produce less ecoli and all plates will have no growth.
Hypothesis was wrong since no ecoli was produced on LB/AMP and LB/AMP-, which means that reducing the heat shock time does not work.